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KMID : 0525720200250030134
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Journal of Chitin and Chitosan
2020 Volume.25 No. 3 p.134 ~ p.142
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Advanced Glycation End-products Inhibitory Activities and Renoprotective Effects of Ishige foliacea Ethanolic Extract
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Cho Chi-Heung
Kim Min-Gyeong
Youm Ga-Hee
Kim Se-Ra
Park Yong-Kon
Lee Sang-Hoon
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Abstract
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The present study was conducted to investigate the anti-glycation activities and renoprotective properties of Ishige foliacea. The yield of extract ranged from 3.20 to 35.59%. Among the extracts, 70% aqueous ethanol extract of Ishige foliacea (70% EtOH IFE) showed the highest total phenolic, condensed tannin, and total flavonoid contents (1.570 ± 0.165 mg GAE/g DW, 0.407 ± 0.008 mg CE/g DE, 0.353 ± 0.050 mg CE/g DE, respectively). 70% EtOH IFE showed the highest breaking activity against preformed AGEs-BSA cross-link than ALT-711, the well-known cross-link breaker. In addition, 70% EtOH IFE observedthe highest inhibitory effect on the AGEs-BSA cross-link and exhibited more potent than aminoguanidine, a well-known AGEs-BSA cross-link inhibitor. Treatment with the 70% EtOH IFE, at 2, 10, and 50 μg/mL, reduced oxidative stress to the level of approximately 186.3-95.9%. The intracellular antioxidative effect of the 70% EtOH IFE was stronger to that with amino-guanidine at 500 μM. Pre-treatments of cells with the 70% EtOH IFE at concentrations of 2, 10, and 50 μg/mL led to 60.3%, 38.0%, and 20.8% decrease in apoptotic cells, respectively. These result suggest that 70% aqueous ethanol extract of Ishige foliacea can serve as a good source of natural anti-diabetic complications agents via breaking and inhibition of AGEs-BSA cross-link and renoprotective effects against MGO-induced oxidative stress.
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KEYWORD
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Ishige foliacea, Advanced glycation end-product, Apoptosis, Condensed tannin, Marine algae
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